EVIDENCE LEDGER / REVIEWED AUGUST 2026

Receipts for the map.

Sources, calculations, and credits for the Connectome Atlas.

32 CATALOG RECORDS15 CORE PAPERS11 CREDIT LINES
SELECTED RENDERS / AMY STERLING

The atlas in pictures.

Connectomics renders by Amy Sterling, made from public reconstruction data.

01 / PRIMARY LITERATURE

Core scientific references.

Primary papers behind the atlas, grouped by research area.

01

FlyWire & FAFB

Whole-brain reconstruction, annotation, analysis and community proofreading

5 REFERENCES
FW01FlyWire reconstruction

Dorkenwald, S. et al. Neuronal wiring diagram of an adult brain. Nature 634, 124–138 (2024).

10.1038/s41586-024-07558-y↗
FW02FlyWire annotations

Schlegel, P. et al. Whole-brain annotation and multi-connectome cell typing of Drosophila. Nature 634, 139–152 (2024).

10.1038/s41586-024-07686-5↗
FW03FlyWire graph analysis

Lin, A., Yang, R. et al. Network statistics of the whole-brain connectome of Drosophila. Nature 634, 153–165 (2024).

10.1038/s41586-024-07968-y↗
FW04FAFB connectivity data

FlyWire Consortium. FlyWire Whole-brain Connectome Connectivity Data, release 783.0. Zenodo (2024).

10.5281/zenodo.10676866↗
FW05Community proofreading

Dorkenwald, S. et al. FlyWire: online community for whole-brain connectomics. Nature Methods 19, 119–128 (2022).

10.1038/s41592-021-01330-0↗
02

Proofreading infrastructure

3D merge/split operations, collaboration, edit history and versioned annotations

2 REFERENCES
PF013D proofreading operations

Zhao, T. et al. NeuTu: Software for Collaborative, Large-Scale, Segmentation-Based Connectome Reconstruction. Frontiers in Neural Circuits 12, 101 (2018).

10.3389/fncir.2018.00101↗
PF02Versioned proofreading & annotation

Dorkenwald, S. et al. CAVE: Connectome Annotation Versioning Engine. Nature Methods 22, 1112–1120 (2025).

10.1038/s41592-024-02426-z↗
03

Whole-animal connectomes

Foundational nervous-system maps across model organisms

4 REFERENCES
CE01C. elegans

White, J. G., Southgate, E., Thomson, J. N. & Brenner, S. The structure of the nervous system of the nematode Caenorhabditis elegans. Phil. Trans. R. Soc. B 314, 1–340 (1986).

10.1098/rstb.1986.0056↗
CE02C. elegans, both sexes

Cook, S. J. et al. Whole-animal connectomes of both Caenorhabditis elegans sexes. Nature 571, 63–71 (2019).

10.1038/s41586-019-1352-7↗
CI01Ciona larva

Ryan, K., Lu, Z. & Meinertzhagen, I. A. The CNS connectome of a tadpole larva of Ciona intestinalis. eLife 5, e16962 (2016).

10.7554/eLife.16962↗
DL01Drosophila larva

Winding, M. et al. The connectome of an insect brain. Science 379, eadd9330 (2023).

10.1126/science.add9330↗
04

Mammalian cortex

Synapse-resolution mouse and human cortical volumes

2 REFERENCES
MI01MICrONS

MICrONS Consortium. Functional connectomics spanning multiple areas of mouse visual cortex. Nature 640, 435–447 (2025).

10.1038/s41586-025-08790-w↗
H001Human cortex / H01

Shapson-Coe, A. et al. A petavoxel fragment of human cerebral cortex reconstructed at nanoscale resolution. Science 384, eadk4858 (2024).

10.1126/science.adk4858↗
05

Field methods & modelling

Connectomics methods and embodied circuit simulation

2 REFERENCES
NM01Field overview

Marx, V. Method of the Year: EM connectomics. Nature Methods 22, 2470–2475 (2025).

10.1038/s41592-025-02906-w↗
SIM1Embodied modelling

Lobato-Rios, V. et al. NeuroMechFly, a neuromechanical model of adult Drosophila melanogaster. Nature Methods 19, 620–627 (2022).

10.1038/s41592-022-01466-7↗
02 / DATA SOURCE REGISTRY

Where each catalog record points.

Project portals are used when a resource is living or versioned; DOI papers are used for fixed publication releases.

Version rule. “Last reviewed” means the catalog links and displayed figures were checked in August 2026. It does not freeze the linked portal. Before quoting a number, cite the project’s named release or materialization.

03 / IMAGE, SOFTWARE & COMMUNITY CREDITS

The map has many makers.

02Interface system

amyleesterling/scifi-ui, adapted for the Connectome Atlas

03Hero and social preview

Connectome rendering supplied by Amy Sterling / What Is a Brain?

04Serial EM and segmentation teaching media

EyeWire / Seung Lab educational materials; each pipeline view links to its source

05MICrONS viewer imagery

MICrONS Explorer and its public Neuroglancer scenes

06Dataset timeline figures

Cook et al.; Ryan, Lu & Meinertzhagen; Scheffer et al.; Winding et al.; Dorkenwald et al.; Shapson-Coe et al.; Qiu et al.; and the MICrONS Explorer

07Program timeline imagery

NIH BRAIN CONNECTS (diagram adapted by Connectome Atlas), Brain/MINDS Data Portal / RIKEN, Chinese Academy of Sciences, and EBRAINS / siibra

08Nature Methods December 2025 cover

Image: Amy Sterling, FlyWire and Princeton University. Cover design: Thomas Phillips. © Springer Nature

09FAFB reconstruction and annotations

FlyWire Consortium, Princeton Neuroscience Institute, Cambridge/MRC LMB researchers and the FlyWire community

10Volume-viewing software

Neuroglancer, Google Research

11Public proofreading context

EyeWire and EyeWire II communities

CORRECTIONS WELCOME

This is a living scholarly object. If a count, citation, release label or credit is incomplete, send the exact correction and its source.

Submit a correction ↗
04 / CALCULATIONS & DEFINITIONS

How the displayed numbers were made.

Reproducible arithmetic where possible; plain disclosure where the interface is illustrative.

01 / FAFB microcircuit total83 + 58 + 50 + 46 + 42 = 279 synapses

The five visible edge weights are the first five rows in the official fafbseg v783 connectivity example for root 720575940603231916. They are individual directed neuron-pair weights, summed only for the small educational graph.

Inspect basis ↗
02 / Connections are not synapses3,732,460 directed connections ≠ ~50,000,000 chemical synapses

Codex counts qualifying directed neuron-to-neuron edges in its FAFB graph. The FlyWire paper reports individual chemical synapses. Many synapses can contribute to one edge; Codex uses a default minimum of five synapses for FAFB connections.

Inspect basis ↗
03 / Catalog record count23 micro records + 9 macro records = 32 catalog entries

This is a count of hand-curated records displayed by this site, not a scientific estimate of every connectomics dataset ever produced. Subvolumes and successive releases can overlap.

Inspect basis ↗
04 / Pipeline section readoutdisplayed z = round(slider depth × 19.79)

The z number, registration confidence, pass counters and proofread score in the pipeline are explanatory interface simulations. They are not measurements derived from the shown microscopy images.

Inspect basis ↗